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KMID : 0359320160560020103
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Korean Journal of Veterinary Research
2016 Volume.56 No. 2 p.103 ~ p.108
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Evaluation of antibacterial and therapeutic effects of egg-white lysozyme against Salmonella Typhimurium in ICR mice infected with Salmonella Typhimurium
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Kim Hee-Gyu
Hwang Jae-Seung
Jae Woo-Young
Son Song-Ee
Lee Hu-Jang
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Abstract
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Salmonellosis is a major bacterial zoonosis that causes self-limited enteritis in animals and foodborne disease and typhoid fever in humans. Recently, multi-drug-resistant strains of Salmonella spp. have increased and caused more serious problems in public health. The present study investigated the antibacterial effects of egg-white lysozyme (EWL) against Salmonella (S.) Typhimurium and the therapeutic effects of EWL for murine salmonellosis. Evaluation of the antibacterial effects of EWL against S. Typhimurium revealed a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of EWL of 6.25 and 300 μg/mL, respectively. In the bacterial growth inhibition test, EWL at 300 (p < 0.05) and 600 μg/mL (p < 0.01) significantly inhibited the growth of S. Typhimurium at 4 h postincubation. EWL administration at MIC (LYS-1), MBC (LYS-2) and 2× MBC (LYS-3) for 14 days resulted in mortality of mice infected with S. Typhimurium of 70, 40 and 10%, respectively, while that of control mice (CON) was 90%. Counts of S. Typhimurium in murine spleens were significantly lower in LYS-2 and LYS-3 than CON (p < 0.05). The results of this study indicate that EWL has the potential for treatment of ICR mice infected with S. Typhimurium.
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KEYWORD
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ICR mouse, egg-white lysozyme, salmonellosis, therapeutic effect
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